An α-carbonic anhydrase from the thermophilic bacterium Sulphurihydrogenibium azorense is the fastest enzyme known for the CO2 hydration reaction

Viviana De Luca; Daniela Vullo; Andrea Scozzafava; Vincenzo Carginale; Mosè Rossi; Claudiu T Supuran; Clemente Capasso

doi:10.1016/j.bmc.2012.09.047

An α-carbonic anhydrase from the thermophilic bacterium Sulphurihydrogenibium azorense is the fastest enzyme known for the CO2 hydration reaction

Bioorg Med Chem. 2013 Mar 15;21(6):1465-9. doi: 10.1016/j.bmc.2012.09.047. Epub 2012 Sep 29.

Authors

Viviana De Luca¹, Daniela Vullo, Andrea Scozzafava, Vincenzo Carginale, Mosè Rossi, Claudiu T Supuran, Clemente Capasso

Affiliation

¹ CNR, Istituto di Biochimica delle Proteine (IBP), Via P. Castellino 111, 80131 Napoli, Italy.

PMID: 23078755
DOI: 10.1016/j.bmc.2012.09.047

Abstract

We report the biochemical characterization of a new carbonic anhydrase (CA, EC 4.2.1.1), named SazCA, identified by translated genome inspection in Sulfurihydrogenibium azorense, a thermophilic bacterium from terrestrial hot springs of the Azores. SazCA is an α-CA showing kinetic parameters that make it the fastest enzyme of the CA family described so far. The biochemical properties, thermostability and inhibition of SazCA were compared with those of the thermophilic and mesophilic counterparts, demonstrating the special features of this unique enzyme.

MeSH terms

Amino Acid Sequence
Bacteria / enzymology*
Carbon Dioxide / metabolism*
Carbonic Anhydrases / classification
Carbonic Anhydrases / metabolism*
Humans
Kinetics
Molecular Sequence Data
Phylogeny
Sequence Alignment
Temperature
Water / metabolism*

Substances

Water
Carbon Dioxide
Carbonic Anhydrases